Skip to main content


eCommons@Cornell

eCommons@Cornell >
Cornell University Graduate School >
Theses and Dissertations (OPEN) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1813/11582
Title: Nanofabricated Fluid Filled Channels for Analysis of Single DNA Molecules
Authors: Mannion, John
Keywords: nanofluidic
nanochannel
DNA
single molecule
entropic force
Issue Date: 3-Nov-2008
Abstract: Nanofabricated, fluid filled channels are presented as tools for the manipulation and detection of single DNA molecules. Both the conformation and position of DNA molecules within such devices can be controlled using a combination of electrophoretic and entropic forces. Application of these forces allows for fluorescently labeled molecules to be stretched in the channel, visualized using fluorescence microscopy, and analyzed in a number of ways, providing valuable and biologically relevant information about a DNA sample. In addition to investigating fluorescence microscopy as a means for sensing molecules in the channel, the possibility of electrical detection is explored. To this end, a fabrication method is described which allows for the integration of single walled carbon nanotube sensors and nanofluidic channels. While this device is currently being tested, it is intended that the incorporated carbon nanotubes may be used in a field effect transistor mode with the goal of detecting perturbations in local solution potential caused by DNA molecules as they are driven through the channels.
URI: http://hdl.handle.net/1813/11582
Appears in Collections:Theses and Dissertations (OPEN)

Files in This Item:

File Description SizeFormat
Mannion_Dissertation_2008-10-30.pdf9.1 MBAdobe PDFView/Open

Refworks Export

Items in eCommons are protected by copyright, with all rights reserved, unless otherwise indicated.

 

© 2014 Cornell University Library Contact Us