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Please use this identifier to cite or link to this item: http://hdl.handle.net/1813/17513
Title: Electroworming : The behaviors of Caenorhabditis (C.) elegans in DC and AC electric fields
Authors: Chuang, Han-Sheng
Raizen, David
Dabbish, Nooreen
Bau, Haim
Keywords: C. elegans
nematode
dielectrophoresis
microfluidics
Issue Date: 15-Oct-2010
Abstract: The video showcases how C. elegans worms respond to DC and AC electrical stimulations. Gabel et al (2007) demonstrated that in the presence of DC and low frequency AC fields, worms of stage L2 and larger propel themselves conscientiously and deliberately towards the cathode. Rezai et al (2010) have demonstrated that this phenomenon, dubbed electrotaxis, can be used to control the motion of worms. In the video, we reproduce Rezai's experimental results. Furthermore, we show, for the first time, that worms can be trapped with high frequency, nonuniform electric fields. We studied the effect of the electric field on the nematode as a function of field intensity and frequency and identified a range of electric field intensities and frequencies that trap worms without apparent adverse effect on their viability. Worms tethered by dielectrophoresis (DEP) avoid blue light, indicating that at least some of the nervous system functions remain unimpaired in the presence of the electrical field. DEP is useful to dynamically confine nematodes for observations, sort them according to size, and separate dead worms from live ones.
URI: http://hdl.handle.net/1813/17513
Appears in Collections:Fluid Dynamics Videos

Files in This Item:

File Description SizeFormat
Electroworming_LD.mpgC. elegans in DC/AC electric fields (640x480 pixels) 8.94 MBMPEGView/Open
Electroworming_HD.mpgC. elegans in DC/AC electric fields (1024x768 pixels)14.37 MBMPEGView/Open

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