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Title: Microfabrication Of Hydrogel Substrates Of Tunable Young'S Modulus
Authors: Charest, Jonathan
Keywords: microfabrication
Youngs modulus
Issue Date: 31-Jan-2012
Abstract: Much is known about the effects of matrix stiffness and matrix topography on cell behavior, however they are seldom investigated in tandem. Presented and validated here is a micromolding method to introduce topographical features onto polyacrylamide (PA) substrates of tunable Young's modulus (E ). Feature fidelity is largely influenced by hydrogel swelling and most efficient on gels with a crosslinker concentration above 4.5%. However, this swelling does not affect the spacing of surface patterned ligands on two-dimensional PA gels. Bovine aortic endothelial cells (BAECs) cultured on linear micromolded features exhibit greater degrees of contact guidance with greater substrate E . Also described are applications of micromolding for future studies in cell mechanics.
Committee Chair: King, Cynthia A.
Committee Member: Bonassar, Lawrence
Chu, Chih-Chang
Discipline: Biomedical Engineering
Degree Name: M.S. of Biomedical Engineering
Degree Level: Master of Science
Degree Grantor: Cornell University
No Access Until: 2017-06-01
Appears in Collections:Cornell Theses and Dissertations

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