Single-molecule, single-cell imaging of stress response systems in Gram-negative bacteria
| dc.contributor.author | Zhang, Wenyao | |
| dc.contributor.chair | Chen, Peng | en_US |
| dc.contributor.committeeMember | Cerione, Richard | en_US |
| dc.contributor.committeeMember | Baird, Barbara | en_US |
| dc.date.accessioned | 2024-04-05T18:48:44Z | |
| dc.date.issued | 2023-08 | |
| dc.description | 220 pages | en_US |
| dc.description.abstract | The study of bacterial stress response systems is an important area of research with wide-ranging implications, from understanding the basic bacterial physiology to developing new antimicrobial treatments. This dissertation presents my recent efforts to understand how Gram-negative bacteria adapt to environmental stimuli using live cell fluorescence imaging, genetic engineering, bulk biochemical/cellular assays, and microfluidic mechanical manipulations. Employing these methods, I, in collaboration with others, investigated three types of stress response systems associated with the Gram-negative bacteria cell envelope: multidrug efflux pumps (MEPs), two-components signal transduction systems (TCSs), and metal homeostasis. | en_US |
| dc.description.embargo | 2025-09-05 | |
| dc.identifier.doi | https://doi.org/10.7298/yq70-mf23 | |
| dc.identifier.other | Zhang_cornellgrad_0058F_13947 | |
| dc.identifier.other | http://dissertations.umi.com/cornellgrad:13947 | |
| dc.identifier.uri | https://hdl.handle.net/1813/114821 | |
| dc.language.iso | en | |
| dc.subject | Biomechanics | en_US |
| dc.subject | Cell-to-cell communication | en_US |
| dc.subject | Multidrug efflux pumps | en_US |
| dc.subject | Protein interactions | en_US |
| dc.subject | Super-resolution imaging | en_US |
| dc.subject | Two-component systems | en_US |
| dc.title | Single-molecule, single-cell imaging of stress response systems in Gram-negative bacteria | en_US |
| dc.type | dissertation or thesis | en_US |
| dcterms.license | https://hdl.handle.net/1813/59810.2 | |
| thesis.degree.discipline | Chemistry and Chemical Biology | |
| thesis.degree.grantor | Cornell University | |
| thesis.degree.level | Doctor of Philosophy | |
| thesis.degree.name | Ph. D., Chemistry and Chemical Biology |
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