Protein Thiocarboxylates: Proteomics, Mechanistic Studies And Pathway Discovery
| dc.contributor.author | Krishnamoorthy, Kalyanaraman | en_US |
| dc.date.accessioned | 2010-08-05T16:21:18Z | |
| dc.date.available | 2015-08-05T06:22:47Z | |
| dc.date.issued | 2010-08-05T16:21:18Z | |
| dc.description.abstract | Protein thiocarboxylates are members of sulfur transfer protein family and have been shown to be involved in a variety of important biosynthetic pathways like vitamin B1, molybdopterin, cysteine, thionucleosides among many others. Despite their importance, there has been lack of systematic efforts towards identifying new thiocarboxylate-forming proteins. In this work, we have taken efforts in developing two strategies to label them in bacterial cell-free extracts using fluorescent tags. In addition, bioinformatics search for new thiocarboxylate-forming proteins using a genomic database, theseed.uchicago.edu and a protein database, Pfam, yielded a new methionine biosynthetic pathway that involves a protein thiocarboxylate as the sulfur donor to make the precursor, homocysteine. This discovery further validated the need for developing methods to identify, in cell-free extracts, proteins carrying this important post-translational modification. The sulfur source for the protein thiocarboxylate involved in the methionine biosynthetic pathway has also been identified as sulfate or sulfite. | en_US |
| dc.identifier.other | bibid: 6980408 | |
| dc.identifier.uri | https://hdl.handle.net/1813/17151 | |
| dc.language.iso | en_US | en_US |
| dc.title | Protein Thiocarboxylates: Proteomics, Mechanistic Studies And Pathway Discovery | en_US |
| dc.type | dissertation or thesis | en_US |
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